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Separation of N-0437 enantiomers by RP-HPLC after precolumn derivatization with D( )-glucuronic acid
Summary
Type:
HPLC
Application #:
105650
Catalog#:
720120.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x150mm
Detection:
UV, 200nm
Conditions:
eluent: water - acetonitrile (91:9) + 0.005M NH3 flow rate 1.0ml/min
Authors:
Gerding TK
Source:
HRC 10 (1987) 523 - 525
Details
Legend: Fig. . Separation of the diastereomeric glucuronic acid conjugates of N-0437 racemate (bottom), (-) enantiomer (middle) and (+)enantiomer (top), analysis of 50µl supernatant by isocratic elution. F
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